trifectUserMessage

billToLabel

nonTrifectaUserMessage

requireAuthentication

unValidatedMessage

unregisteredMessage

requireRegistration

requiresValidation

unauthenticatedMessage

siteLink

siteLogo

headerLinks

Header Links

parentCompanyLink

mainSiteLink

footerLinks

Footer Links

copyrights

opcoLogos

Opco Logos

trademarks

parentCompanyLogo

mainSiteLinkText

regulatory

siteRobotsTXT

gtmId

footerGTMScript

headerGTMScript

Content Source Notes

MAPSS Code

Alert banners

isExternal

description

title

style

checkCommerceEnabled

Copyright

Legal

Online Terms of Use

Patents

Privacy Statement

Sitemap

Danaher Life Sciences

Dashboard

Quotes

Scheduled Orders

Order History

order-center

redeemQuotePage

Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells. The Ki-67 antigen is a human nuclear protein defined by its reactivity with monoclonal antibody from the Ki-67 clone. Two isoforms of 345 and 395 kDa have been identified by cDNA sequence coding. Deduced amino acid sequence analysis of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. The Ki-67 antigen is expressed at active phases of the cell cycle (G1, S, G2 and M phases), but it is absent in resting cells (G0 phase). The level of Ki-67 antigen expression varies during cell cycle and it has been correlated to several cell pathways: The Ki-67 decrease pathway is characterized by a declining Ki-67 staining and leads eventually to the exit from the active cell cycle (G0). If cells on this pathway get stimulated by growth factors, they can enter the Ki-67 increase pathway that brings the cells back into S phase. Cells following the Ki-67 stable pathway exhibit a constant intensity of Ki-67 staining during the G1 phase. This pathway is thought to correspond to optimal local growth conditions. The cellular localization of the Ki-67 protein is cell cycle phase dependent. During interphase, the antigen can be exclusively detected within the nucleus, whereas in M phase most of the protein is relocated to the surface of the chromosomes. The antigen is rapidly degraded as the cell enters the non proliferative state.

Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells. The Ki-67 antigen is a human nuclear protein defined by its reactivity with monoclonal antibody from the Ki-67 clone. Two isoforms of 345 and 395 kDa have been identified by cDNA sequence coding. Deduced amino acid sequence analysis of the Ki-67 antigen confirmed that the cDNA encodes for a nuclear and short-lived protein without any significant homology to known sequences. The Ki-67 antigen is expressed at active phases of the cell cycle (G1, S, G2 and M phases), but it is absent in resting cells (G0 phase). The level of Ki-67 antigen expression varies during cell cycle and it has been correlated to several cell pathways: The Ki-67 decrease pathway is characterized by a declining Ki-67 staining and leads eventually to the exit from the active cell cycle (G0). If cells on this pathway get stimulated by growth factors, they can enter the Ki-67 increase pathway that brings the cells back into S phase. Cells following the Ki-67 stable pathway exhibit a constant intensity of Ki-67 staining during the G1 phase. This pathway is thought to correspond to optimal local growth conditions. The cellular localization of the Ki-67 protein is cell cycle phase dependent. During interphase, the antigen can be exclusively detected within the nucleus, whereas in M phase most of the protein is relocated to the surface of the chromosomes. The antigen is rapidly degraded as the cell enters the non proliferative state.

*Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc.

B68180, Ki-67-AF488 Conjugated Antibody, Ki-67, 0.5 mL, ASR

Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle (G1, S, G2, M phases). It is never expressed by resting (G0) cells.

Learn more about this product on Beckman.com

Ki-67-Alexa Fluor 488, Ki-67, 0.5 mL, ASR